Characterization of the respiratory chain from cultured Crithidia fasciculata
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چکیده
منابع مشابه
Characterization of the respiratory chain from cultured Crithidia fasciculata.
Mitochondrial mRNAs encoding subunits of respiratory-chain complexes in kinetoplastids are post-transcriptionally edited by uridine insertion and deletion. In order to identify the proteins encoded by these mRNAs, we have analyzed respiratory-chain complexes from cultured cells of Crithidia fasciculata with the aid of 2D polyacrylamide gel electrophoresis (PAGE). The subunit composition of F0F1...
متن کاملCharacterization of recombinant glutathionylspermidine synthetase/amidase from Crithidia fasciculata.
Trypanothione [N1,N8-bis(glutathionyl)spermidine] is a unique metabolite found only in trypanosomatids, where it subsumes many of the functions of GSH in other organisms. In Crithidia fasciculata, two distinct ATP-dependent ligases, glutathionylspermidine synthetase (GspS; EC 6.3.1.8) and trypanothione synthetase (TryS; EC 6.3.1.9), are involved in the synthesis of trypanothione from GSH and sp...
متن کاملIsolation and characterization of drug resistant mutants of Crithidia fasciculata.
Mutants of Crithidia fasciculata, resistant to Actinomycin D, Tubercidin, Crystal Violet, 6-Azauridine, and 5-Fluorouracil were obtained and characterized. The mutants were stable when maintained under nonselective conditions, and could be grouped in several subclasses on the basis of cross-resistance to other drugs. Actinomycin D- and Crystal Violet-resistant mutants appeared spontaneously whe...
متن کاملThe growth and nutrition of Crithidia fasciculata.
The conditions for luxuriant axenic cultivation of the trypanosomid flagellate Crithidia fasciculata in chemically-defined media were determined. Improvements over the hitherto published media include higher concentrations of amino acids, folic acid (where this is the sole source of pteridine) and purines and the inclusion of threonine and Tween 80. Increased oxygenation was obtained by incubat...
متن کاملPurification and characterization of DNA ligase I from the trypanosomatid Crithidia fasciculata.
A DNA ligase has been purified approximately 5000-fold, to near homogeneity, from the trypanosomatid Crithidia fasciculata. The purified enzyme contains polypeptides with molecular masses of 84 and 80 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Both polypeptides formed enzyme-adenylate complexes in the absence of DNA, contained an epitope that is highly conser...
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ژورنال
عنوان ژورنال: Molecular and Biochemical Parasitology
سال: 1997
ISSN: 0166-6851
DOI: 10.1016/s0166-6851(96)02823-x